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grna cloning vector px330  (Addgene inc)


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    Structured Review

    Addgene inc grna cloning vector px330
    Grna Cloning Vector Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+cloning+vector+px330/pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1%2F110)+(Plasmid+%2371707)/pmc10935061-46-1-5
    Average 93 stars, based on 22 article reviews
    grna cloning vector px330 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates.
    Article Snippet: .. Briefly, the design of single-guide RNAs (sgRNAs) was based on recommendations from the Zhang laboratory website (http://crispr.mit.edu/). gRNA targeting sequence of Nur77 (5′-ACCTTCATGGACGGCTACAC-3′) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway
    Article Snippet: .. Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’-GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates
    Article Snippet: .. Briefly, the design of single-guide RNAs (sgRNAs) was based on recommendations from the Zhang laboratory website ( http://crispr.mit.edu/ ). gRNA targeting sequence of Nur77 (5′-ACCTTCATGGACGGCTACAC-3′) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a Novel Nur77 Modulator, Induces Apoptosis of Cancer Cells by Activating the Nur77-Bcl-2 Apoptotic Pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’- GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’-GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Clone Assay:

    Article Title: Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates.
    Article Snippet: .. Briefly, the design of single-guide RNAs (sgRNAs) was based on recommendations from the Zhang laboratory website (http://crispr.mit.edu/). gRNA targeting sequence of Nur77 (5′-ACCTTCATGGACGGCTACAC-3′) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway
    Article Snippet: .. Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’-GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates
    Article Snippet: .. Briefly, the design of single-guide RNAs (sgRNAs) was based on recommendations from the Zhang laboratory website ( http://crispr.mit.edu/ ). gRNA targeting sequence of Nur77 (5′-ACCTTCATGGACGGCTACAC-3′) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a Novel Nur77 Modulator, Induces Apoptosis of Cancer Cells by Activating the Nur77-Bcl-2 Apoptotic Pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’- GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’-GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Cloning:

    Article Title: Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates.
    Article Snippet: .. Briefly, the design of single-guide RNAs (sgRNAs) was based on recommendations from the Zhang laboratory website (http://crispr.mit.edu/). gRNA targeting sequence of Nur77 (5′-ACCTTCATGGACGGCTACAC-3′) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway
    Article Snippet: .. Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’-GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates
    Article Snippet: .. Briefly, the design of single-guide RNAs (sgRNAs) was based on recommendations from the Zhang laboratory website ( http://crispr.mit.edu/ ). gRNA targeting sequence of Nur77 (5′-ACCTTCATGGACGGCTACAC-3′) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a Novel Nur77 Modulator, Induces Apoptosis of Cancer Cells by Activating the Nur77-Bcl-2 Apoptotic Pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’- GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’-GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: Phase separation of Nur77 mediates XS561-induced apoptosis by promoting the formation of Nur77/Bcl-2 condensates
    Article Snippet: .. The gRNA cloning vector Px330 (Addgene, 71707) was used for cloning. ..

    Plasmid Preparation:

    Article Title: Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates.
    Article Snippet: .. Briefly, the design of single-guide RNAs (sgRNAs) was based on recommendations from the Zhang laboratory website (http://crispr.mit.edu/). gRNA targeting sequence of Nur77 (5′-ACCTTCATGGACGGCTACAC-3′) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway
    Article Snippet: .. Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’-GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates
    Article Snippet: .. Briefly, the design of single-guide RNAs (sgRNAs) was based on recommendations from the Zhang laboratory website ( http://crispr.mit.edu/ ). gRNA targeting sequence of Nur77 (5′-ACCTTCATGGACGGCTACAC-3′) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a Novel Nur77 Modulator, Induces Apoptosis of Cancer Cells by Activating the Nur77-Bcl-2 Apoptotic Pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’- GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’-GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: Phase separation of Nur77 mediates XS561-induced apoptosis by promoting the formation of Nur77/Bcl-2 condensates
    Article Snippet: .. The gRNA cloning vector Px330 (Addgene, 71707) was used for cloning. ..

    CRISPR:

    Article Title: BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway
    Article Snippet: .. Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’-GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a Novel Nur77 Modulator, Induces Apoptosis of Cancer Cells by Activating the Nur77-Bcl-2 Apoptotic Pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’- GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’-GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Knock-Out:

    Article Title: BI1071, a Novel Nur77 Modulator, Induces Apoptosis of Cancer Cells by Activating the Nur77-Bcl-2 Apoptotic Pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’- GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..

    Article Title: BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway
    Article Snippet: .. Generation of Nur77 and Bcl-2 Knock-out cells by CRISPR/Cas9 system Knocking out Nur77 and Bcl-2 from HeLa cells employed the CRISPR/Cas9 system. gRNA targeting sequence of Nur77 (5’-ACCTTCATGGACGGCTACAC-3’) and Bcl-2 (5’-GAGAACAGGGTACGATAACC-3’) was cloned into gRNA cloning vector Px330 (Addgene, 71707) and confirmed by sequencing. ..



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    Addgene inc grna cloning vector px330
    Grna Cloning Vector Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+cloning+vector+px330/pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1%2F110)+(Plasmid+%2371707)/pmc10935061-46-1-5
    Average 93 stars, based on 1 article reviews
    grna cloning vector px330 - by Bioz Stars, 2026-10
    93/100 stars
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